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三聚氰胺 ELISA检测试剂盒说明书

发布时间:2017-05-31 22:50:45 分类:行业新闻 来源: 点击:次

三聚氰胺 ELISA检测试剂盒说明书 三聚氰胺 ELISA检测试剂盒说明书   1.概述  此试剂盒的原理是酶联免疫测定法,这个试剂盒可用于1切样品中3聚氰胺的定性或定量检测。如果需要进1步测定可以利用HPLC,GC/MS技术。  2.安全指点  试剂盒标准中含有少许的3聚氰胺,另外,底物溶液中含有4甲基联苯胺,终止液里含有稀释的盐酸。尽可能避免不要和这些试剂接触,如果这些试剂接触到皮肤请马上用水冲洗。  3.存储和稳定性  试剂盒应在4–8℃保存,在使用前试剂盒内的所有试剂都要回温到室温(20⑵5℃)在有效期内试剂可以1直使用  4.原理  酶联免疫吸附测定法定量测定3聚氰胺残留。将标准、样品提取物和3聚氰胺酶标记物加入到已包被有3聚氰胺抗体的微孔中开始反应。在30分钟的孵育进程中,样品萃取物中的3聚氰胺与3聚氰胺酶标记物竞争结合微孔中的3聚氰胺抗体,孵育30分钟后洗掉微

  1.GAISHU

爱拼彩票平台  CISHIJIHEDEYUANLISHIMEILIANMIANYIDIANZILIXUESHIYANJICEDINGFA,ZHEIGESHIJIHEKEYONGYU1QIEYANGPINZHONG3JUQINGANDEDINGXINGHUODINGLIANGJIANCE。RUGUOXUYAOJIN1BUCEDINGKEYILIYONGHPLC,GC/MSJISHU。

  2.ANQUANZHIDIAN

爱拼彩票平台  SHIJIHEBIAOZHUNZHONGHANYOUSHAOXUDE3JUQINGAN,LINGWAI,DIWURONGYEZHONGHANYOU4JIAJILIANBENAN,ZHONGZHIYELIHANYOUXISHIDEYANSUAN。JINKENENGBIMIANBUYAOHEZHEIXIESHIJIJIECHU,RUGUOZHEIXIESHIJIJIECHUDAOPIFUQINGMASHANGYONGSHUICHONGXI。

  3.CUNCHUHEWENDINGXING

爱拼彩票平台  SHIJIHEYINGZAIZAIDAIBOLISHIYANJI4–8℃BAOCUN,ZAISHIYONGQIANSHIJIHENEIDESUOYOUSHIJIDOUYAOHUIWENDAOSHIWEN(20⑵5℃)ZAIYOUXIAOQINEISHIJIKEYI1ZHISHIYONG

  4.YUANLI

  MEILIANMIANYIXIFUCEDINGFADING1tDANHUANGPILAOSHIYANJILIANGCEDING3JUQINGANCANLIU。JIANGBIAOZHUN、YANGPINTIQUWUHE3JUQINGANMEIBIAOJIWUJIARUDAOYIBAOBEIYOU3JUQINGANKANGTIDEWEIKONGZHONGKAISHIFANYING。ZAI30FENZHONGDEFUYUJINCHENGZHONG,YANGPINCUIQUWUZHONGDE3JUQINGANYU3JUQINGANMEIBIAOJIWUJINGZHENGJIEHEWEIKONGZHONGDE3JUQINGANKANGTI,FUYU30FENZHONGHOUXIDIAOWEIKONGZHONGSUOYOUWUJIEHEDE3JUQINGANJIFANGZHILALISHIYANJI3JUQINGANMEIBIAOJIWU。ZAIYONGXISHIDEXIYEQINGXIJIESHUHOU,MEIKONGZHONGJIARUQINGCHELUOSINIULISHIYANJIDEDIWURONGYE,JIEHEDEMEIBIAOJIWUJIANGWUSEDEDIWUZHUANHUAWEILANSEDEWUZI。FUYU20FENZHONGHOUTINGZHICIFANYING,GENJUGEKONGSECAISHENQIANJINXINGSHUJUDUQU。GENJUBIAOZHUNDESECAIDECHUYANGPINZHONG3JUQINGANDEDENONGDUZHI。

  5.JUXIANXING

  WOMENYIDUIKENENGCHUXIANZAIJIANCEYANGPINZHONGDEHENDUOYOUJIHEWUJIWUZUOLEJIANCE,FAXIANTAMENBUHEZHEIGESHIJIHECHANSHENGJIAOCHAFANYING。DANSHIZAIYANGPINZHONGYEYOU1XIEYIBIANZHIDEHUAHEWU,TAMENTONGGUOJIZHIYINGXIANGLAIGANRAOCEDING,RUHANZHIFANGDESHIPIN,TAMENZAICEJIAOZHANDAICHIZHANXINGSHIYANJIDINGQIANYAOTONGGUOXISHILAIXIAOCHU1XIEJIZHIYINGXIANG。ZAISHIYONGDEJINCHENGZHONGCHUXIANSHIWUYEHUIYINGXIANGJIEGUO,LIRUSHIJIHECUNCHUDETIAOJIAN、XIQUYETIDECHENGXU、XIQUYETIDEBUZHUNQUE、ZAICHANSHENGMIANYIHEDIWUFANYINGDEJINCHENGZHONGFUYUSHIJIANBUZHUNQUE。

  6.GONGZUOSHUJU

  MINGANXING:3JUQINGANJIANCEXIAN10μg/L,B/B0WEI50%SHIDEZHIWEI150 μg/L,CEDINGJIEGUOZAIBIAOZHUNQUXIANDEZHONGJIANSHIZUIJINGQUEDE。

  ZHONGFUXING:

  BIAOZHUNDEBIANYIXISHU(CVs) <10%;YANGPINDEBIANYIXISHU(CVs)<15%.

  TEYIXING:

  A SHIJIHEZUCHENG

爱拼彩票平台  1、ZHENKONGBAOZHUANGWEIKONGBAN(8×12TIAO),BAOBEIYOU3JUQINGANDUOKELONGKANGTI

爱拼彩票平台  2、6PING3JUQINGANBIAOZHUNYE,NONGDUFENBIEWEI0、20、50、100、200、500ng/mL(ppb)

爱拼彩票平台  3、1PING7mL 3JUQINGANMEIBIAOJIWU

  4、5×NONGSUOXIYE 100ml HEZHONGQUXIANSHIYANJI;SHIYONGQIANXUYAOSHIYONGZHENGLIUSHUIYI1:5DEBILIXISHI

  5、1PING14mLDIWU(TMB)

  6、1 PING14mLZHONGZHIYE.(ZHUYI:YANSUAN,WUJIECHUPIFU!).

  B CESHIQIANZHUNBEI

爱拼彩票平台  WEILIANGYIYEQI" href="http://www.18show.cn/product/detail/12645637.html" target="_blank">WEILIANGYIYEQIHEXITOUSHIBIXUSHIYONGDE,TUIJIANSHIYONGPAIQIANG,ZAIZUOTONG1CICESHISHIYAOSHIYONGTONG1GEHEZILIDESHIJIHEBIAOZHUN。

爱拼彩票平台  1、SHIYONGQIANJIANGSUOYOUDESHIJINADAOSHIWENJIAOZHILIUGAOYALOUDIANQIHENSHIYANJI(19℃⑵5℃)。

爱拼彩票平台  2、CONGLVBODAIZHONGNACHUYAOQIUSHULIANGDEWEIKONGTIAO,FANGRUGANZAOJIBINGZHONGXINFENGHAODAIZIYIBIMIANWEIKONGTIAOSHOUCHAO。ZHIYU4⑻℃BAOCUN。

  3、BIAOZHUNYE,MEIJIEHEWU,DIWU,ZHONGZHIYEBUYONGXISHIZHIJIESHIYONG。

  4、XIYEZAISHIYONGQIANYAOYI1:5DEBILIXISHIHOUSHIYONG(BIRU:10mlXIYE+40mlDEZHENGLIUSHUI)。

  5、YOUYUZHONGZHIYEZHONGBAOKUOYANSUANNADESHIHOUYAOJINSHEN。

  C. BUZHOU

爱拼彩票平台  1、ZAIDUIYINGDEWEIKONGZHONGJIARU100 μL BIAOZHUNHUOYANGPIN。ZUIHAOZUOZHONGFU。

  2、YONGPAIQIANGZAIMEIYIGEKONGZHONGDOUJIARU50μLQIBAOXIANZHENKONGSHIYANJIMEIBIAOJIWU、RANHOUYONGZHIXIANGBAOXINGZOUSHIYANJIPIANGAIZHUWEIKONG,QINGQINGZHENDANGWEIKONGBAN30MIAOSHIKONGZHONGDEYETIHUNYUN。

  3、ZAISHIWENXIAFUYU30FEN。

  4、JIANGKONGLIDEYETIDAORUSHIHEDERONGQIZHONG,MEIYIGEWEIKONGZUISHAOJIARU250μL、1×XIYE,RANHOUQIDIAOXIYEDAOSHIHEDERONGQIZHONG,ZHONGFUXI3CI。ZAI1CENGHOUDEXISHUIZHISHANGPAIBAN,QUDIAOCANLIUDEXIYE。DINGXIANGDIELUOSHIYANJI

  5、SHIYONGPAIQIANGZAIMEIYIGEKONGZHONGJIARU100μLDIWUXIANSEYE,ZAISHIWENFUYU20FEN。JINKENENGSHIQIBUJIANGUANG。

爱拼彩票平台  6、MEIKONGJIARU100μLZHONGZHIYE,BANZHONGSECAIJIANGHUIYOULANSEBIANCHENGZONGHUANGSE。

  7、450nm XIADUQUMEIYIGEKONGDEXIGUANGDUZHI(OD)。

  D. JIEGUOPINGGU

爱拼彩票平台  KEYILIYONGSHANGYEELISARUANJIANCHENGXUBIRULogit/Log or 4-ParameterLAIPINGJIAELISAJIEGUO。YENENGGOUTONGGUOJISUANMEIYIGEBIAOZHUNDE%B/B0ZHI,YI%B/B0WEIyZHOU、3JUQINGANNONGDUWEIxZHOUZUO1BIAOZHUNQUXIAN。YANGPINNONGDUKEYITONGGUOBIAOZHUNQUXIANLAIJISUAN。DANGYANGPINZHONGDE3JUQINGANNONGDUGAOYUBIAOZHUN5(500μg/L)SHI,XUYAOXISHIHOUZHONGXINZAICEDINGLAIQUDEGENGJINGQUEDEJIEGUO。

  BANDINGLIANGJIEGUODEPANDING,KEGENJUYANGPINDEXIGUANGDUZHIYUBIAOZHUNDEXIGUANGDUZHILAIPANDING,YANGPINDEXIGUANGSHUINIHENGYINGLIYALISHIYANJIDUZHIDIYUMOUGEBIAOZHUNDEXIGUANGDUZHI,ZESHUOMINGYANGPINDE3JUQINGANHANLIANGDAYUCIBIAOZHUNDENONGDU,YANGPINDEXIGUANGDUZHIGAOYUMOUGEBIAOZHUNDEXIGUANGDUZHI,ZESHUOMINGYANGPINDE3JUQINGANHANLIANGXIAOYUCIBIAOZHUNDENONGDU。

  E.HAIXUZHUANGBEI

  1、50μL ~200μL DANDAOHUODUODAOYIYEQIHEXITOU

  2、WEIKONGBANXIDIJI

  3、JINGANGSHICHONGJISHIYANJIZHENDANGQI

  4DAOXIANLADUANLISHIFUZAIRANSHAOSHIYANJIYANJI、ZHENGLIUSHUIHUOQULIZISHUI

  5、XISHUIZHI

  6、LIXINJI'>LIXINJI

爱拼彩票平台  7、450nm LVGUANGPIANDEMEIBIAOYI

三聚氰胺 ELISA检测试剂盒说明书